Genotoxic Effects of a-Endosulfan and b-Endosulfan on Human HepG2 Cells

Genotoxic effects of alpha-endosulfan and beta-endosulfan on human HepG2 cells.

alpha-Endosulfan and ss-endosulfan are isomers of endosulfan, a pesticide used worldwide. In this study, we examined the genotoxicity of [alpha]- and ss-endosulfan in vitro with a HepG2 cell line. We used sister chromatid exchanges (SCE), micronuclei (MN), and DNA strand breaks as detected by single-cell gel electrophoresis (SCG) assays as biomarkers to judge the genotoxicity of [alpha]- and ss...

Modulatory Effect of Vitamin C on Genotoxic Effect of Endosulfan in Developing Albino Rats

The genotoxic effect of endosulfan and the modulatory effect of vitamin C in growing albino rats were studied by bone marrow micronucleus assay. Seven days old male Wistar rats were treated with 3, 6, 9, 12mg/kg Endosulfan orally (10 pups/group),for up to 60 days, at intervals of 24h. For 2 more groups (n=10/group), Endosulfan 9mg/kg and 12mg/kg was administered along with vitamin C (20mg/kg). ...

Penicillium sp. as an organism that degrades endosulfan and reduces its genotoxic effects

Endosulfan is an organochloride and persistent pesticide that has caused concern because of its impact in the environment and its toxicity to and bioaccumulation in living organisms. In this study, we isolated an endosulfan-degrading fungus from the activated sludge from an industrial wastewater treatment plant. Through repetitive enrichment and successive subculture in media containing endosul...

Endosulfan and Endocrine Disruption:

The pesticides endosulfan, toxaphene, and dieldrin have estrogenic effects on human estrogen-sensitive cells.

Estrogenic pesticides such as DDT and chlordecone generate deleterious reproductive effects. An "in culture" bioassay was used to assess the estrogenicity of several pesticides. The E-screen test uses human breast estrogen-sensitive MCF7 cells and compares the cell yield achieved after 6 days of culture in medium supplemented with 5% charcoal-dextran stripped human serum in the presence (positi...